贵阳市白云区人民医院;贵州省第二人民医院;贵州医科大学;遵义医科大学;贵州省临床检验中心;
目的 RNA-seq转录组测序分析青少年重度抑郁症(major depression disease, MDD)患者外周血差异表达的lncRNA、miRNA、mRNA,为阐明青少年MDD发病机制提供理论补充。方法 随机收集样本库4例青少年女性MDD患者与4例正常人对照外周血，TRizol试剂处理后，提取RNA后进行RNA-seq转录组测序，根据P<0.05且|Log2FC|>1计算两组样本的表达差异，绘制差异lncRNA、miRNA、mRNA热图与火山图。通过TargetScan数据库可能预测差异miRNA调控的mRNA,并进行GO功能与KEGG信号通路聚类。结果 MDD组和对照组两组间满足P<0.05且|Log2FC|>1差异条件的MDD组中1 636个lncRNA上调和825个lncRNA下调，42个miRNAs上调和30个miRNAs下调，2 469个mRNA上调和1 398个mRNA下调。靶基因主要富集的GO功能：生物学过程(BP)、分子功能(MF)、细胞组分(CC)三个方面。靶基因主要富集的KEGG信号通路：差异表达的lncRNA多富集在Toll-like receptor信号通路、TNF信号通路、Salmonella intection和NF-kappa B信号通路等；差异表达的miRNA多富集在VEGF信号通路、Vasopressin-regulated water reabsorption、Vascular smooth muscle contraction、MAPK信号通路等；差异表达的mRNA靶基因多富集在泛素介导的蛋白质水解、T细胞受体信号通路和MAPK信号通路等。结论 本研究结果提供了青少年女性MDD外周血中lncRNA、miRNA、mRNA表达变化的一般情况与可能调控的功能与通路，可能有助于阐明青少年MDD的潜在发病机制。
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基本信息:
DOI：
中图分类号:R749.4
引用信息:
[1]姜洁,任真奎,刘志国等.RNA-seq转录组测序分析青少年MDD患者外周血lncRNA、miRNA、mRNA表达差异[J].贵州医药,2025,49(02):171-176.
基金信息:
国家自然科学基金(82160311); 贵州省卫生健康委科学技术基金(gzwkj2021-256)